| |
|
|
| Registros recuperados : 24 | |
| 1. |  | CALEGARIO, R. F.; FERREIRA, S. de S.; ANDRADE, E. C. de; ZERBINI, F. M. Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 9, p. 1335-1343, set. 2007 Título em português: Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil.| Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
|    |
| 2. | | CALEGARIO, R. F.; ANDRADE, E. C.; FERREIRA, S. S.; MANHANI, G. G.; ZERBINI, F. M. Biological and molecular characterization of a tomato isolate of Sidra micrantha mosaic vírus (SimMV). Fitopatologia Brasileira, Brasília, DF, v. 30, p. S180, ago. 2006. Suplemento. Resumo 749. Trabalho apresentado no 38. Congresso Brasileiro de Fitopatologia, 2005, Brasília, DF.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 3. | | SOUZA, T. A.; INOUE-NAGATA, A. K.; CALEGARIO, R. F.; KITAJIMA, E. W. Identificatio brugmansia saveolens mottle virus in brugnansia SP. Virus Reviews and Research, v. 20, n. 2, p. 138, 2016. Edição dos resumos do XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 4. | | CALEGARIO, R. F.; ALFENAS, P. F.; COTRIM, M. A.; ANDRADE, E. C.; ZERBINI, F. M. Caracterization of a new tomato-infecting begomovirus and its potencial use a vector for transient gene expression in plants. Virus Reviews & Research, Florianópolis, v. 8, p. 193-194, 2003. Resumo. Trabalho apresentado no 14º National Meeting of Virology, Florianópolis, 2003.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 6. | | AMBROZEVÍCIUS, L. P.; ANDRADE, E. C.; CALEGARIO, R. F.; FONTES, E. P. B.; ZERBINI, F. M. Molecular cloning and characterization of TGV-Ig, a tomato-infecting Begomovirus from Igarapé, Minas Gerais State. Virus Reviews & Research, Florianópolis, v. 5, n. 2, p. 196, 2000. Suplemento 1. Resumo. Trabalho apresentado no 11º National Meeting of Virology,São Lourenço, 2000.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 7. | | AMBROZEVICIUS, L. P.; CALEGARIO, R. F.; FONTES, E. P. B.; CARVALHO, M. G. de; ZERBINI, F. M. Genetic diversity of begomovirus infecting tomato and asscociated weeds in Southeastern Brazil. Fitopatologia Brasileira, Brasília, DF, v. 27, n. 4, p. 372-377, jul/ago. 2002.| Biblioteca(s): Embrapa Hortaliças. |
|  |
| 8. | | GARITA, L. C.; TASSI, A. D.; CALEGARIO, R. F.; KITAJIMA, E. W.; CARBONELL, S. A. M.; ASTUA, J. de F. Common bean: experimental indicator plant for Citrus leprosis virus C and some other Cytoplasmic-type brevipalpus-transmitted viruses. Plant Disease, v. 97, n. 10, p. 1346, 2013.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 9. | | CALEGARIO, R. F.; AMBROZEVICIUS, L. P.; SILVEIRA, S. F.; RODRIGUES, I. L.; ZAMBOLIM, E. M.; ZERBINI, F. M. Survey of tomato geminivirus in Rio de Janeiro State Brazil. Fitopatologia Brasileira, Brasilia, v.25, p.438, ago. 2000. Suplemento. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 10. | | KITAJIMA, E. W.; CALEGARIO, R. F.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; BASTIANEL, M.; FREITAS-ASTÚA, J.; FRANCISCHINI, F. In situ detection of CiLV-C in the mite vector Brevipalpus phoenicis, evidence for a circulative type of virus/vector relationship and a model for the virus circulation in the mite. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 11. | | KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. 1 CD ROM| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 12. | | ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; NUNES, M. A.; JESUS, C. C.; CALEGARIO, R. F.; KITAJIMA, E. W.; NOVELLI, V. M.; ASTUA, J. de F. Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses. Scientia Agricola, v.74, n.1, p.1-10X, 2017| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 13. | | GARITA, L. C.; TASSI, A. D.; CALEGARIO, R. F.; ASTUA, J. de F.; SALAROLI, R. B.; ROMÃO, G. O.; KITAJIMA, E. W. Experimental host range of Citrus leprosis virus C (CiLV-C). Tropical Plant Pathology, v. 39, n.1, p.043-055, 2014.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 14. | | ZERBINI, F. M.; AMBROZEVICIUS, L. P.; ANDRADE, E. C.; BARROS, D. R.; CALEGARIO, R. F.; FERNANDES, J. J.; BROMMOSCHENKEL, S. H.; FONTES, E. P. B.; CARVALHO, M. G. Genetic diversity of novel begomovirus species infecting tomatoes and associated weeds in Brazil. Virus Reviews & Research, Florianópolis, v. 8, p. 32-33, 2003. Resumos. Trabalhos apresentado no 14º National Meeting of Virology, Florianópolis, 2003.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 15. | | KITAJIMA, E. W.; CHAGAS, C. M.; HARAKAVA, R.; CALEGARIO, R. F.; ASTUA, J. de F.; RODRIGUES, C. V.; CHILDERS, C. C. Citrus leprosis in Florida, USA, appears to have been caused by the nuclear type of Citrus Leprosis Virus (CiLV-N). Virus Reviews and Research, January 2011.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 16. | | NUNES, M. A.; LAMEIRO, P.; CALEGARIO, R. F.; BERGAMINI, M. P.; COERINI, L. F.; KITAJIMA, E. W.; BASTIANEL, M.; NOVELLI, V. M.; ASTUA, J. de F. Trapoeraba (Commelina benghalensis L.) como fonte de inóculo do vírus da leprose dos citros. Citrus Research & Technology, Cordeirópolis, v.33, n.1, p.1-9, 2012.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 17. | | KITAJIMA, E. W.; RAMOS-GONZÁLEZ, P. L.; ASTUA, J. de F.; ALEXANDRE, M. A. V.; PIRES, G. R. R.; CHABI-JESUS, C.; TASSI, A. D.; CALEGARIO, R. F. Novas espécies de plantas hospedeiras naturalmente infectadas por vírus transmitidos por Ácaros Brevipalpus no Brasil new plant host species naturally infected by brevipalpus-transmitted viruses in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 174. Evento online.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 18. | | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; TASSI, A. D.; CALEGARIO, R. F.; HARAKAVA, R.; NOME, C F.; KITAJIMA, E. W.; ASTUA, J. de F. A Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae. Frontiers in Microbiology, v.28, n.13, 836076, March 2022.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 19. | | CALEGARIO, R. F.; LOCALI, E. C.; STACH-MACHADO, D. R.; PERON, L. A.; CASERTA, R.; SALAROLI, R. B.; ASTUA, J. de F.; MACHADO, M. A.; KITAJIMA, E. W. Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: production and use in immunodiagnosis. Tropical Plant Pathology, v.38 n.3 Brasília May/June 2013. Epub May 14, 2013.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 20. | | CALEGARIO, R. F.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; SALAROLI, R. B.; FREITAS-ASTÚA, J.; STACH- MACHADO, D. R.; CASERTA, R.; MACHADO, M. A. In situ immunocytochemistry of citrus leaves infected with Citrus leprosis virus cytoplasmic type (CiLV-C). Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| Registros recuperados : 24 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
25/10/2012 |
|
Data da última atualização: |
31/10/2012 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
CALEGARIO, R. F.; LABATE, M. T. V.; PERONI, LUÍS A.; STACH-MACHADO, D. R.; ASTUA, J. de F.; LABATE, C. A.; MACHADO, M. A.; KITAJIMA, E. W. |
|
Afiliação: |
RENATA FAIER CALEGARIO, ESALQ; MÔNICA TERESA VENEZIANO LABATE, ESALQ; LUÍS ANTÔNIO PERONI, UNICAMP; DAGMAR RUTH STACH-MACHADO, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF; CARLOS ALBERTO LABATE, ESALQ; MARCOS ANTÔNIO MACHADO, Centro APTA Citros Sylvio Moreira; ELLIOT WATANABE KITAJIMA, ESALQ. |
|
Título: |
In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C). |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Tropical Plant Pathology, n. 2, v. 37, p. 255-265, March-April, 2012. |
|
ISSN: |
1982-5676 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Citrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous protein expressed in vitro by ELISA, but poorly with the native protein present in leaf lesion extracts from sweet orange caused by CiLV-C. Reactions from old lesions were more intense than those from young lesions. Western blot and in situ immunolocalization assays failed to detect the native protein. These results suggest low expression of the movement protein (MP) in host tissues. Moreover, it is possible that the conformation of the protein expressed in vitro and used to produce the antibody differs from that of the native MP, hindering a full recognition of the latter. Key words: Brevipalpus phoenicis, Cilevirus, Citrus sinensis, polyclonal antibody, serology. |
|
Palavras-Chave: |
Polyclonal antibody. |
|
Thesagro: |
Brevipalpus Phoenicis; Citrus Sinensis. |
|
Thesaurus NAL: |
Cilevirus; Citrus; Citrus leprosis virus C; serology. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/68741/1/in-vitro-expression.pdf
|
|
Marc: |
LEADER 02278naa a2200301 a 4500
001 1937928
005 2012-10-31
008 2012 bl uuuu u00u1 u #d
022 $a1982-5676
100 1 $aCALEGARIO, R. F.
245 $aIn vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C).
260 $c2012
520 $aCitrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous protein expressed in vitro by ELISA, but poorly with the native protein present in leaf lesion extracts from sweet orange caused by CiLV-C. Reactions from old lesions were more intense than those from young lesions. Western blot and in situ immunolocalization assays failed to detect the native protein. These results suggest low expression of the movement protein (MP) in host tissues. Moreover, it is possible that the conformation of the protein expressed in vitro and used to produce the antibody differs from that of the native MP, hindering a full recognition of the latter. Key words: Brevipalpus phoenicis, Cilevirus, Citrus sinensis, polyclonal antibody, serology.
650 $aCilevirus
650 $aCitrus
650 $aCitrus leprosis virus C
650 $aserology
650 $aBrevipalpus Phoenicis
650 $aCitrus Sinensis
653 $aPolyclonal antibody
700 1 $aLABATE, M. T. V.
700 1 $aPERONI, LUÍS A.
700 1 $aSTACH-MACHADO, D. R.
700 1 $aASTUA, J. de F.
700 1 $aLABATE, C. A.
700 1 $aMACHADO, M. A.
700 1 $aKITAJIMA, E. W.
773 $tTropical Plant Pathology$gn. 2
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
